The X-Gel Spotter can complete the fully automatic dispensing of matrigel in 96-well/384-well plates, effectively solving the inefficiency, inconsistency and technical bottlenecks of traditional manual operation, thereby promoting the standardization, high-throughput quantification and precision of organoid research.
Defects of manual dispensing: When manually laying matrigel (such as Matrigel), the volume, shape, and thickness are difficult to control, resulting in large differences in the organoid growth microenvironment, and the experimental results fluctuate significantly (e.g., the difference in organoid size can reach more than 50% under the same conditions).
The X-Gel Spotter dispenses liquids with minimal precision through I-omics Technology's unique high-precision micropump, ensuring that the diameter, height, and cell density of each "droplet" are highly consistent, improving experimental reproducibility.
The automated sampler can complete the droplet array of 384-well plates in less than 10 minutes, support 24/7 continuous operation, and adapt to robotic integration systems to meet the high-throughput needs of pharmaceutical companies or core facilities (such as screening 5,000 compounds per day).
Through the multi-channel spot head, heterogeneous droplet arrays (such as with different ECM components and growth factor gradients) can be programmed to simulate the spatial heterogeneity of the tumor microenvironment and study cell-to-cell communication or drug resistance.
One-click operation, even non-professionals can master it within 2 hours, reducing the dependence of the laboratory on senior technicians.
The sampler can be directly connected to the automated incubator and high-content imaging system to realize the full closed-loop automation of "droplet dispensing-culture-monitoring-dosing", such as using it in conjunction with the system to complete the AI tracking of organoid growth curves.
Organ research is shifting from "workshop" to "industrialization", and manual operations have become bottlenecks in data quality, scale expansion, and clinical translation. Matrix glue samplers are like "PCR instruments in organoids", transforming experimental reliability from "art" to "engineering" and are a critical infrastructure for standardized processes approved by FDA, such as the FDA's Draft Organoid Guidance.
Technical parameters
It can complete matrix glue dots in 96-well/384-well plates;
It can complete the addition of cell culture medium or cell fluid change in the culture plate.
Speed: < 2.5 min / 96-well plate (single channel) ; < 10 min/384 well (single channel); The speed can be increased by 8 times with 8-channel pipelines;
Type: 6-384-well plates;
100uL dispensing volume accuracy: CV<2%;
The flow speed is adjustable in multiple stages, which is conducive to different cell aliquoting or media addition, and the slow flow rate can be used for media addition to prevent the risk of matrix glue being washed away.
Complex hole unequal volume dispensing can be imported through Excel table (with computer and software);
With a low-temperature incubation module, the matrix glue can be kept at a low temperature;
It can be integrated with high connotation, incubator, etc. into a fully automatic all-in-one machine;
Dimensions: 375mm(W) x 383mm(D) x 250mm(H);
Model:
X-Gel Spotter: Standard model, can be sampled according to the direction of the column or row, and can select any column for spotting;
X-Gel Plus Spotter (with computer and control software): control the single-channel steel needle into any hole for dispensing through the computer and control software, and can import the Excel table to perform different micro-wells or different volumes of each hole according to the table requirements.
